- B.A. with honors, 1976, University of Chicago
- Ph.D., 1982, Stanford University, microbiology.
- Department of Biology faculty, 1984-
Joined the department in 2004.
Honors and appointments:
Speaker-elect of Faculty Senate
ASM International Professorship
Regents Professor (2012)
Dean of Faculty and Associate Provost
College Station, TX 77843-3258
Biological Sciences Building East
Biological Sciences Building East
|Bacterial Molecular Genetics and Biotechnology
Dr. Benedik is not currently accepting new students.
My laboratory studies basic biological problems using molecular genetic methods with simple microbial systems. Additionally we are developing novel microbial approaches for biotechnological applications.
Biotechnology/Bioremediation - Cyanide
The aim of this project is better enzymes for degrading cyanide in waste streams and contaminated sites. The industrial uses of cyanide have resulted in contamination at many sites, especially the water and soil of metal plating plants and as the result of ore extraction in mining operations. Especially in light of recent highly-publicized incidents of cyanide contamination (e.g., in Houston-area plating facilities as well as the recent cyanide release in Eastern Europe) there is a need to develop lower-cost, efficient methods to detoxify these sites. Cyanide is a common constituent of biological systems and is actually produced by a variety of organisms, especially plants. Due to the toxicity of cyanide, nature has evolved numerous biochemical pathways for its conversion to innocuous byproducts. There is previous work on biodegradation of cyanide, either by enzymes or metabolically-active whole cells, using a variety of different pathways. The most interesting for our project are those cyanide-degrading enzymes which can function without the need for active cellular metabolism, and can be used under conditions which would kill microorganisms.
We hope to apply modern molecular biology to improve the cost, stability, and metal-tolerance of cyanidases, enzymes which convert cyanide directly to formate and ammonia. These end products are vastly less toxic than cyanide, and they can also be directly metabolized by indigenous microorganisms to cell mass, CO2, and water. Like other enzymes, cyanidases are capable of scavenging and destroying their substrate (i.e., cyanide) down to extremely low levels (< 0.01 ppm). Cyanidases have already been applied to cyanide removal, but the commercial technology is relatively old, and has not taken advantage of: (1) the ability to overexpress enzyme activities in alternative hosts, (2) opportunities for functional improvement by protein engineering, and (3) the discovery and cloning (by others in the literature) of new forms of this enzyme with potentially superior properties.
Our work is leading to further insights on the structural biology of this important branch of nitrilase enzymes as well as in the development of novel enzymes for industrial applications in bioremedation.
Bacterial Persistence and Antibiotic Tolerance
Antibiotics are effective at killing most bacteria, however for any large population there are always a small number of survivors no matter how long the treatment is administered. These bacterial cells that survive antibiotic treatment are called persisters and the phenomenon of bacterial persistence is long-standing. The project aims to elucidate the molecular mechanisms of dormancy in bacteria where bacteria are not killed by antibiotics but rather persist throughout treatment and awaken after the antibiotic therapy ceases. This leads to disease recurrence as well as increased antimicrobial resistance. The project aims to understand the signals that lead to the dormant state and eventually to develop therapeutic interventions that can “wake” these bacteria before antibiotic therapy ceases.
- Kwan BW, Osbourne DO, Hu Y, Benedik MJ & Wood TK (2014) Phosphodiesterase DosP increases persistence by reducing cAMP which reduces the signal indole. Biotechnol Bioeng 0: Full text
- Vilo C, Benedik MJ, Ilori M & Dong Q (2014) Erratum for Vilo et al., Draft Genome Sequence of Cupriavidus sp. Strain SK-3, a 4-Chlorobiphenyl- and 4-Chlorobenzoic Acid-Degrading Bacterium. Genome Announc 2: Full text
- Hu Y, Kwan BW, Osbourne DO, Benedik MJ & Wood TK (2014) Toxin YafQ increases persister cell formation by reducing indole signalling. Environ Microbiol 2: Full text
- Vilo C, Benedik MJ, Ilori M & Dong Q (2014) Draft Genome Sequence of Cupriavidus sp. Strain SK-3, a 4-Chlorobiphenyl- and 4-Clorobenzoic Acid-Degrading Bacterium. Genome Announc 2: Full text
- Vilo C, Benedik MJ, Ilori M & Dong Q (2014) Draft Genome Sequence of Cupriavidus sp. Strain SK-4, a di-ortho-Substituted Biphenyl-Utilizing Bacterium Isolated from Polychlorinated Biphenyl-Contaminated Sludge. Genome Announc 2: Full text
- Guo Y, Quiroga C, Chen Q, McAnulty MJ, Benedik MJ, Wood TK & Wang X (2014) RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res 42:6448-62 Full text
- Cheng HY, Soo VW, Islam S, McAnulty MJ, Benedik MJ & Wood TK (2014) Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress. Environ Microbiol 16:1741-54 Full text
- Martínez AK, Gordon E, Sengupta A, Shirole N, Klepacki D, Martinez-Garriga B, Brown LM, Benedik MJ, Yanofsky C, Mankin AS, Vazquez-Laslop N, Sachs MS & Cruz-Vera LR (2014) Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan. Nucleic Acids Res 42:1245-56 Full text
- Ilori MO, Picardal FW, Aramayo R, Adebusoye SA, Obayori OS & Benedik MJ (2013) Catabolic plasmid specifying polychlorinated biphenyl degradation in Cupriavidus sp. strain SK-4: Mobilization and expression in a pseudomonad. J Basic Microbiol 42:1245-56 Full text
- Kwan BW, Valenta JA, Benedik MJ & Wood TK (2013) Arrested protein synthesis increases persister-like cell formation. Antimicrob Agents Chemother 57:1468-73 Full text