- B.A. with honors, 1976, University of Chicago
- Ph.D., 1982, Stanford University, microbiology.
- Department of Biology faculty, 1984-
Joined the department in 2004.
Honors and appointments:
Speaker-elect of Faculty Senate
ASM International Professorship
Regents Professor (2012)
Dean of Faculty and Associate Provost
College Station, TX 77843-3258
Biological Sciences Building East
Biological Sciences Building East
|Bacterial Molecular Genetics and Biotechnology
Dr. Benedik is not currently accepting new students.
My laboratory studies basic biological problems using molecular genetic methods with simple microbial systems. Additionally we are developing novel microbial approaches for biotechnological applications.
Biotechnology/Bioremediation - Cyanide
The aim of this project is better enzymes for degrading cyanide in waste streams and contaminated sites. The industrial uses of cyanide have resulted in contamination at many sites, especially the water and soil of metal plating plants and as the result of ore extraction in mining operations. Especially in light of recent highly-publicized incidents of cyanide contamination (e.g., in Houston-area plating facilities as well as the recent cyanide release in Eastern Europe) there is a need to develop lower-cost, efficient methods to detoxify these sites. Cyanide is a common constituent of biological systems and is actually produced by a variety of organisms, especially plants. Due to the toxicity of cyanide, nature has evolved numerous biochemical pathways for its conversion to innocuous byproducts. There is previous work on biodegradation of cyanide, either by enzymes or metabolically-active whole cells, using a variety of different pathways. The most interesting for our project are those cyanide-degrading enzymes which can function without the need for active cellular metabolism, and can be used under conditions which would kill microorganisms.
We hope to apply modern molecular biology to improve the cost, stability, and metal-tolerance of cyanidases, enzymes which convert cyanide directly to formate and ammonia. These end products are vastly less toxic than cyanide, and they can also be directly metabolized by indigenous microorganisms to cell mass, CO2, and water. Like other enzymes, cyanidases are capable of scavenging and destroying their substrate (i.e., cyanide) down to extremely low levels (< 0.01 ppm). Cyanidases have already been applied to cyanide removal, but the commercial technology is relatively old, and has not taken advantage of: (1) the ability to overexpress enzyme activities in alternative hosts, (2) opportunities for functional improvement by protein engineering, and (3) the discovery and cloning (by others in the literature) of new forms of this enzyme with potentially superior properties.
Our work is leading to further insights on the structural biology of this important branch of nitrilase enzymes as well as in the development of novel enzymes for industrial applications in bioremedation.
Bacterial Persistence and Antibiotic Tolerance
Antibiotics are effective at killing most bacteria, however for any large population there are always a small number of survivors no matter how long the treatment is administered. These bacterial cells that survive antibiotic treatment are called persisters and the phenomenon of bacterial persistence is long-standing. The project aims to elucidate the molecular mechanisms of dormancy in bacteria where bacteria are not killed by antibiotics but rather persist throughout treatment and awaken after the antibiotic therapy ceases. This leads to disease recurrence as well as increased antimicrobial resistance. The project aims to understand the signals that lead to the dormant state and eventually to develop therapeutic interventions that can “wake” these bacteria before antibiotic therapy ceases.
- Martínez AK, Gordon E, Sengupta A, Shirole N, Klepacki D, Martinez-Garriga B, Brown LM, Benedik MJ, Yanofsky C, Mankin AS, Vazquez-Laslop N, Sachs MS & Cruz-Vera LR (2013) Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan. Nucleic Acids Res 0: Full text
- Ilori MO, Picardal FW, Aramayo R, Adebusoye SA, Obayori OS & Benedik MJ (2013) Catabolic plasmid specifying polychlorinated biphenyl degradation in Cupriavidus sp. strain SK-4: Mobilization and expression in a pseudomonad. J Basic Microbiol 0: Full text
- Kwan BW, Valenta JA, Benedik MJ & Wood TK (2013) Arrested protein synthesis increases persister-like cell formation. Antimicrob Agents Chemother 57:1468-73 Full text
- Wang X, Lord DM, Hong SH, Peti W, Benedik MJ, Page R & Wood TK (2013) Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS. Environ Microbiol 15:1734-44 Full text
- Vilo CA, Benedik MJ, Kunz DA & Dong Q (2012) Draft genome sequence of the cyanide-utilizing bacterium Pseudomonas fluorescens strain NCIMB 11764. J Bacteriol 194:6618-9 Full text
- Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R & Wood TK (2012) A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nat Chem Biol 8:855-61 Full text
- Hong SH, Wang X, O'Connor HF, Benedik MJ & Wood TK (2012) Bacterial persistence increases as environmental fitness decreases. Microb Biotechnol 5:509-22 Full text
- Martínez AK, Shirole NH, Murakami S, Benedik MJ, Sachs MS & Cruz-Vera LR (2012) Crucial elements that maintain the interactions between the regulatory TnaC peptide and the ribosome exit tunnel responsible for Trp inhibition of ribosome function. Nucleic Acids Res 40:2247-57 Full text
- Hu Y, Benedik MJ & Wood TK (2012) Antitoxin DinJ influences the general stress response through transcript stabilizer CspE. Environ Microbiol 14:669-79 Full text
- Wang L, Watermeyer JM, Mulelu AE, Sewell BT & Benedik MJ (2012) Engineering pH-tolerant mutants of a cyanide dihydratase. Appl Microbiol Biotechnol 94:131-40 Full text