Faculty: James Smith 2016-12-15T11:18:34+00:00
James Smith

James Smith

Associate Professor

Fax: 979-845-2891
Email: jsmith@bio.tamu.edu

Curriculum Vitae
Smith Lab Website

Office:
3258 TAMU
Biological Sciences Building East
Room 314D
979-845-2417

Lab:
Biological Sciences Building East
Rooms 318 and 320
979-845-2446

Joined the Department in 2010

  • B.S., 1996, Florida State University, Biology and Secondary Science Math Teaching.
  • M.B.A., 2002, University of Florida,Gainesville, Finance/Competitive Strategy.
  • Ph.D. 2002, University of Florida, Gainesville, Biochemistry/Microbiology.
  • Postdoctoral research: Oragenics Inc.
  • Prior faculty appointment: Mississippi State University. 

Discovery and Characterization of Novel Antimicrobial Agents

The discovery of novel antimicrobials and the study of antimicrobial function have significant relevance towards the development of therapeutics aimed at treating life threatening diseases. However, much of what we have learned about protein synthesis, DNA replication, enzyme function, as well as membrane physiology comes from the study of antimicrobials. Our knowledge of cellular and membrane physiology is still limited and there is much we need to learn. The discovery and structural and functional characterization of new antimicrobial agents will provide new insights into cellular and membrane function, as well as provide means to intellectually design new analogs that target microbial function. The discovery of new enzymes involved in natural product synthesis also provides invaluable information in understanding the complexity of microorganisms and provides tools for synthetic chemistry applications.

  1. Escano, J, Ravichandran, A, Salamat, B, Smith, L. Carboxyl Analogue of Mutacin 1140, a Scaffold for Lead Antibacterial Discovery. Appl. Environ. Microbiol. 2017;83 (14):. doi: 10.1128/AEM.00668-17. PubMed PMID:28500042 PubMed Central PMC5494638.
  2. Deng, P, Foxfire, A, Xu, J, Baird, SM, Jia, J, Delgado, KH et al.. The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14. Appl. Environ. Microbiol. 2017;83 (8):. doi: 10.1128/AEM.00051-17. PubMed PMID:28188204 PubMed Central PMC5377494.
  3. Ortega, MA, Cogan, DP, Mukherjee, S, Garg, N, Li, B, Thibodeaux, GN et al.. Two Flavoenzymes Catalyze the Post-Translational Generation of 5-Chlorotryptophan and 2-Aminovinyl-Cysteine during NAI-107 Biosynthesis. ACS Chem. Biol. 2017;12 (2):548-557. doi: 10.1021/acschembio.6b01031. PubMed PMID:28032983 PubMed Central PMC5315687.
  4. Deng, P, Wang, X, Baird, SM, Showmaker, KC, Smith, L, Peterson, DG et al.. Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis. Microbiologyopen. 2016;5 (3):353-69. doi: 10.1002/mbo3.333. PubMed PMID:26769582 PubMed Central PMC4905989.
  5. Escano, J, Smith, L. Multipronged approach for engineering novel peptide analogues of existing lantibiotics. Expert Opin Drug Discov. 2015;10 (8):857-70. doi: 10.1517/17460441.2015.1049527. PubMed PMID:26004576 .
  6. Escano, J, Stauffer, B, Brennan, J, Bullock, M, Smith, L. Biosynthesis and transport of the lantibiotic mutacin 1140 produced by Streptococcus mutans. J. Bacteriol. 2015;197 (7):1173-84. doi: 10.1128/JB.02531-14. PubMed PMID:25605307 PubMed Central PMC4352662.
  7. Escano, J, Stauffer, B, Brennan, J, Bullock, M, Smith, L. The leader peptide of mutacin 1140 has distinct structural components compared to related class I lantibiotics. Microbiologyopen. 2014;3 (6):961-72. doi: 10.1002/mbo3.222. PubMed PMID:25400246 PubMed Central PMC4263518.
  8. Emrick, D, Ravichandran, A, Gosai, J, Lu, S, Gordon, DM, Smith, L et al.. The antifungal occidiofungin triggers an apoptotic mechanism of cell death in yeast. J. Nat. Prod. 2013;76 (5):829-38. doi: 10.1021/np300678e. PubMed PMID:23672235 .
  9. Chen, S, Wilson-Stanford, S, Cromwell, W, Hillman, JD, Guerrero, A, Allen, CA et al.. Site-directed mutations in the lanthipeptide mutacin 1140. Appl. Environ. Microbiol. 2013;79 (13):4015-23. doi: 10.1128/AEM.00704-13. PubMed PMID:23603688 PubMed Central PMC3697549.
  10. Chen, KC, Ravichandran, A, Guerrero, A, Deng, P, Baird, SM, Smith, L et al.. The Burkholderia contaminans MS14 ocfC gene encodes a xylosyltransferase for production of the antifungal occidiofungin. Appl. Environ. Microbiol. 2013;79 (9):2899-905. doi: 10.1128/AEM.00263-13. PubMed PMID:23435879 PubMed Central PMC3623131.
Search PubMed