• B.S., 1982, Southern Methodist University, Biology.
  • Ph.D., 1987, Indiana University, Genetics.
  • Postdoctoral research, Brandeis University.
  • Department of Biology faculty, 1991-1995.
  • Prior faculty appointments at Texas A&M (1991-1995) and University of Houston (1995-2005)

Joined the department in 2005.

John W. Lyons Jr. ’59 Chair in Biology.
Center for Biological Clocks Research (Director)
Faculty of Genetics
Texas A&M Institute for Neuroscience

Hardin Lab Webpage

Molecular Genetics of Biological Clocks

A diverse array of organisms including prokaryotic and eukaryotic microbes, plants, and animals display daily rhythms in physiology, metabolism and/or behavior. These rhythms are not passively driven by environmental cycles of light and temperature, but are actively controlled by endogenous circadian clocks that are set by environmental cycles, keep time in the absence of environmental cues, and activate overt physiological, metabolic and behavioral rhythms at the appropriate time of day. This remarkable conservation of circadian clock function through evolution suggests that maintaining synchrony with the environment is of fundamental importance. Our understanding of the circadian clock is particularly important for human health and well-being. The clearest examples of circadian clock dysfunction are those that result in abnormal sleep-wake cycles, but clock disturbances are also associated with other ailments including epilepsy, cerebrovascular disease, depression, and seasonal affective disorder. The realization that disorders of the sleep-wake cycle such as Familial Advanced Sleep Phase Syndrome can result from alterations in clock gene function underscores the clinical importance of understanding the molecular organization of the circadian system.

Work in my laboratory focuses on defining the molecular mechanisms that drive circadian clock function in the fruit fly, Drosophila melanogaster. We previously found that the core timekeeping mechanism is based on core and interlocked transcriptional feedback loops. Our studies currently focus on (1) defining post-translational regulatory mechanisms that operate in the core loop to set the 24 hour period, (2) determining whether interlocked loops are important for circadian timekeeping and/or output, (3) understanding how circadian oscillator cells are determined during development, and (4) defining mechanisms that control rhythms in olfactory and gustatory physiology and behavior.

  1. Agrawal, P, Hardin, PE. The Drosophila Receptor Protein Tyrosine Phosphatase LAR Is Required for Development of Circadian Pacemaker Neuron Processes That Support Rhythmic Activity in Constant Darkness But Not during Light/Dark Cycles. J. Neurosci. 2016;36 (13):3860-70. doi: 10.1523/JNEUROSCI.4523-15.2016. PubMed PMID:27030770 PubMed Central PMC4812141.
  2. Liu, T, Mahesh, G, Houl, JH, Hardin, PE. Circadian Activators Are Expressed Days before They Initiate Clock Function in Late Pacemaker Neurons from Drosophila. J. Neurosci. 2015;35 (22):8662-71. doi: 10.1523/JNEUROSCI.0250-15.2015. PubMed PMID:26041931 PubMed Central PMC4452561.
  3. Zhou, J, Yu, W, Hardin, PE. ChIPping away at the Drosophila clock. Meth. Enzymol. 2015;551 :323-47. doi: 10.1016/bs.mie.2014.10.019. PubMed PMID:25662463 .
  4. Lee, E, Jeong, EH, Jeong, HJ, Yildirim, E, Vanselow, JT, Ng, F et al.. Phosphorylation of a central clock transcription factor is required for thermal but not photic entrainment. PLoS Genet. 2014;10 (8):e1004545. Epub 2014/8/14. doi: 10.1371/journal.pgen.1004545. PubMed PMID:25121504 PubMed Central PMC4133166.
  5. Glossop, NR, Gummadova, JO, Ghangrekar, I, Hardin, PE, Coutts, GA. Effects of TWIN-OF-EYELESS on Clock Gene Expression and Central-Pacemaker Neuron Development in Drosophila. J. Biol. Rhythms. 2014;29 (3):151-166. doi: 10.1177/0748730414534819. PubMed PMID:24916389 PubMed Central PMC4262727.
  6. Mahesh, G, Jeong, E, Ng, FS, Liu, Y, Gunawardhana, K, Houl, JH et al.. Phosphorylation of the transcription activator CLOCK regulates progression through a ∼ 24-h feedback loop to influence the circadian period in Drosophila. J. Biol. Chem. 2014;289 (28):19681-93. doi: 10.1074/jbc.M114.568493. PubMed PMID:24872414 PubMed Central PMC4094078.
  7. Menet, JS, Hardin, PE. Circadian clocks: the tissue is the issue. Curr. Biol. 2014;24 (1):R25-7. doi: 10.1016/j.cub.2013.11.016. PubMed PMID:24405673 PubMed Central PMC4157344.
  8. Hardin, PE, Panda, S. Circadian timekeeping and output mechanisms in animals. Curr. Opin. Neurobiol. 2013;23 (5):724-31. doi: 10.1016/j.conb.2013.02.018. PubMed PMID:23731779 PubMed Central PMC3973145.
  9. Kaneko, H, Head, LM, Ling, J, Tang, X, Liu, Y, Hardin, PE et al.. Circadian rhythm of temperature preference and its neural control in Drosophila. Curr. Biol. 2012;22 (19):1851-7. doi: 10.1016/j.cub.2012.08.006. PubMed PMID:22981774 PubMed Central PMC3470760.
  10. Hardin, PE. Molecular genetic analysis of circadian timekeeping in Drosophila. Adv. Genet. 2011;74 :141-73. doi: 10.1016/B978-0-12-387690-4.00005-2. PubMed PMID:21924977 PubMed Central PMC4108082.
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Paul Hardin

Paul E. Hardin
Distinguished Professor

3258 TAMU
College Station, TX 77843-3258

Office:
Biological Sciences Building West
308A
979-458-4478

Lab:
Biological Sciences Building West
308
979-845-0382

Fax: 979-845-2891
Email: phardin@bio.tamu.edu

Curriculum Vitae