Welcome to Golden Labs Holiday Party 12/05/07!
Our Goals
To assay the function of each gene of the S. elongatus PCC 7942 genome through gene inactivation, with a focus on the phenotypes associated with the circadian rhythmicity of gene expression.
To determine the complete genome sequence.
To make these data available to the scientific community as soon as possible through publicly accessible databases.
Project News Dec 07 The TIGR (now JCVI) version of the automated annotation of the S. elongatus PCC 7942 genome is available at TIGR’s CMR (Comprehensive Microbial Resource) online database. Aug 06 We have performed saturation mutagenesis on approximately 92% of the chromosome from 77 cosmids or JGI plasmid sets. Sequencing of the transposon insertions on the 45 cosmids is completed. JGI plasmid sets are being sequenced to fill the gaps left. A JGI plasmid set is defined as a pool of several plasmids from the JGI 8-kb library that was used to generate the genome, and represents approximately 40-kb of contiguous genomic DNA. Mar 06 A web-based Manatee server for manual annotation is now up and running for S. elongatus PCC 7942. Please contact us if you are interested in curation of your favorite genes of the automated annotation from The Institute for Genomic Research (TIGR). We will be very glad to send you the user name and password, as well as other relevant documents. For detailed information about this tool, please go to the homepage of the Manatee project, which is supported by TIGR. Dec 05 The JGI version of automated annotation of S. elongatus PCC 7942 is now available on both GenBank (CP000100) and the Integrated Microbial Genomes (IMG) system at JGI. Aug 05 DNA Research has published our strategy and current status paper of the functional genome project of S. elongatus PCC 7942 (Holtman et al, 2005). Apr 05 The complete sequence and automated annotation of S. elongatus PCC 7942 genome is now available. For current status of the shotgun sequencing project, please go to the homepage of Synechococcus elongatus PCC 7942 at JGI Microbes. Feb 05 The complete sequence and curated annotation of Synechococcus elongatus PCC 6301, a strain closely related to S. elongatus PCC 7942, is now available in GenBank (AP008231). The chromosome of PCC 6301 (2,696,225 bp) is slightly longer than that of PCC 7942 (2,695,903 bp). A single large inversion (~189 kb, see the chromosome map for its position) accounts for the few detectable RFLPs between the two genomes (Golden et al, 1989). PCC 7942 is naturally transformable, while PCC 6301 is not. Aug 04 Synechococcus elongatus PCC 7942 has been chosen as a target for draft sequencing by the DOE Joint Genome Institute (JGI)! We will coordinate our project with the JGI shotgun sequence to complete functional genomics and annotated sequence as quickly as possible. To avoid duplication of effort, we will no longer close and annotate individual cosmids. Rather, our sequencing will be limited to localizing the transposon insertions, and we will focus on gene inactivation in the cyanobacterium.
