Zachary W. Culumber

PhD student, Rosenthal lab

Department of Biology and Interdisciplinary Research Group in Ecology & Evolutionary Biology, Texas A&M University

Centro de Investigaciones Científicas de las Huastecas “Aguazarca”

Curriculum vitae

 

Education: B.S. with High Distinction - University of Illinois (Urbana) – Kim Hughes (Advisor), Undergraduate Thesis: “No associations between an MHC class-II gene and parasite resistance in guppies (Poecilia reticulata)”

 

Research Interests: Evolutionary genetics of sexual selection, population genetics, mechanisms maintaining genetic variation (particularly adaptive genetic variation such as MHC genes)

 

I have broad interests in the areas of behavior, ecological and evolutionary genetics, particularly as they pertain to mate choice and sexual selection, and a general interest in the mechanisms maintaining genetic variation in natural populations.  The current focus of my dissertation is an investigation of population genetics in Xiphophorus hybrid zones of eastern Mexico and of the evolutionary genetics of sexual selection in this system.

 

Single nucleotide polymorphism (SNP) genotyping and DNA sequencing of a limited number of loci have shown significant spatial variation in population structure among hybrid zones found along replicated ecological gradients in at least six stream reaches.  The goal of my dissertation is to understand how genetic variation is distributed along the hybrid zones and how patterns are similar or different across hybrid zones and to characterize the role of female mate choice in structuring populations.

 

Mating simulations on current SNP data suggest that different mating patterns account for variation in population structure in different populations.  By combining analyses of behavior, genetics and theory I can better determine what processes are determining population structure and the role of female mate choice.  To accomplish this I am conducting mate choice assays in the lab using animated playback experiments, genotyping embryos to infer mate genotypes and modeling mating patterns with computer simulations.  Together these areas can show how females are mating in the wild, how closely this resembles their actual preferences as determined in the lab and the contribution of their mating preferences to population structure in hybrid populations.

 

Soon genotyping of hundreds of single nucleotide polymorphisms (SNP) derived from express sequence tags (EST) will enable interval mapping to indicate chromosomal areas associated with female choice.  From this I can determine how genetic variation underlying female choice contributes to variation in mating patterns and population structure.  Multilocus SNP genotyping can also allow us to construct genetic clines to determine what loci are under selection and how patterns of selection vary among hybrid zones.

 

I am also currently an NSF IGERT trainee through the interdisciplinary Applied Biodiversity Science program at Texas A&M, and associated with the Ecology and Evolutionary Biology group at A&M.